Meet the Authors: Single-cell Multiomics Analysis of Clonal Evolution in Myeloid Malignancies

This webinar, the first in a “Meet the Authors” series sponsored by Mission Bio, discusses the application of single-cell analysis to decipher clonal evolution across several stages of disease development in myeloid malignancies.

Our invited speaker, Dr. Ross Levine of Memorial Sloan Kettering Cancer Center, discusses his study published in Nature, “Single-cell mutation analysis of clonal evolution in myeloid malignancies,” which provides insights into the pathogenesis of myeloid transformation and how clonal complexity evolves with disease progression.

As part of this work, Dr. Levine’s lab performed single-cell DNA sequencing on 146 samples from 123 patients with clonal hematopoiesis, myeloproliferative neoplasms, and/or acute myeloid leukemia to delineate mutations at single clone resolution and map out the clonal complexity of each indication. Furthermore, they combined cell-surface protein expression with mutational analysis to correlate genotype and immunophenotype.

Dr. Levine talks about the benefits of using single-cell analysis to elucidate clonal evolution, including the ability to distinguish which mutations occur in the same clone(s), accurately measure clonal complexity, and definitively determine the order of mutations.