In pursuit of the leukemic stem cell: leveraging single-cell genomics and proteomics to identify drivers of AML

Dr. Cathy Smith from UCSF provides a case study on single-cell sequencing of polyclonal resistance to FLT3 inhibitors in acute myeloid leukemia (AML). She presents data taken over a period of 28, 48, and 92 days to reveal that NRAS clones were detectable in 0.1% of cells with single-cell analysis at 28 days, which was previously undetectable with bulk sequencing until relapse.