Women in Single Cell: Single-Cell Analysis and Genome Editing in Preclinical Disease Modeling: Detailed Insights into Leukemia Lineage

This webinar, the first in a “Women in Single Cell” series sponsored by Mission Bio, discusses the use of single-cell analysis to assess genome editing for use in pre-clinical disease modeling.

Our invited speaker, Ilaria Iacobucci of St. Jude Children’s Research Hospital, discusses the benefits of using single-cell analysis to quantify and characterize genome editing experiments.

In particular, she discusses a study she co-authored that was published in Blood, “Modeling and targeting of erythroleukemia by hematopoietic genome editing,” in which she developed faithful experimental models of acute erythroid leukemia (AEL) in mice by multiplex genome editing of recurrently co-mutated genes.

As part of this work, Dr. Iacobucci and colleagues used single-cell analysis to determine mutational co-occurrence and sequence of acquisition of multiple tumor stages, identifying primary and secondary mutations that promote clonal fitness and genesis of the leukemic phenotype.