Assessment of Whole-Genome Copy Number Variation with Tapestri Single-Cell Analysis

Copy number variants (CNVs) are a prevalent form of genetic variation in cancer and serve as both a key safety metric in cell and gene therapy development and a biomarker for cancer progression. Conventional CNV assays either provide bulk averages of genomic gains and losses or offer single-cell measurements with insufficient breadth and throughput for comprehensive analysis. In this technical note, we present the Tapestri Genome Integrity CNV Solution, a robust assay that identifies CNV-based subclones with high sensitivity and specificity. This assay enables comprehensive characterization of CNV signatures in cells, providing a powerful tool for both safety assessments and disease biology investigations.