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Mission Bio is committed to helping you learn about single-cell DNA sequencing and multi-omics and how these exciting technologies are advancing medical science. Enjoy exploring our education hub!

References

  1. Nowell P. The clonal evolution of tumor cell populations. Science. 1976;194:23-28.
  2. Shaughnessy J. Amplification and overexpression of CKS1B at chromosome band 1q21 is associated with reduced levels of p27Kip1 and an aggressive clinical course in multiple myeloma. Hematology. 2005;10 Suppl 1:117-26.
  3. Landau DA et al. Clonal evolution in hematological malignancies and therapeutic implications. Leukemia. 2014 Jan;28(1):34-43.
  4. Morita K et al. Clonal evolution of acute myeloid leukemia revealed by high-throughput single-cell genomics. Nat Commun. 2020 Oct 21;11(1):5327.
  5. Miles LA et al. Single-cell mutation analysis of clonal evolution in myeloid malignancies. Nature. 2020 Nov;587(7834):477-482.
  6. Demaree B et al. Joint profiling of proteins and DNA in single cells reveals extensive proteogenomic decoupling in leukemia. bioRxiv 2020.02.26.967133.
  7. App Note: Single-cell DNA analysis with nuclei isolated from solid tumor tissue. Mission Bio.
  8. Gao Y et al. V211D Mutation in MEK1 Causes Resistance to MEK Inhibitors in Colon Cancer. Cancer Discov. 2019 Sep;9(9):1182-1191. Epub 2019 Jun 21.
  9. de Bruin EC et al. Intra-tumor heterogeneity: lessons from microbial evolution and clinical implications. Genome Med. 2013 Nov 22;5(11):101.
  10. Bochtler T et al. Clonal heterogeneity as detected by metaphase karyotyping is an indicator of poor prognosis in acute myeloid leukemia. J Clin Oncol. 2013 Nov 1;31(31):3898-905.
  11. Maia C et al. Biological and clinical significance of dysplastic hematopoiesis in patients with newly diagnosed multiple myeloma. Blood. 2020 Jun 25;135(26):2375-2387.
  12. Albert -Servera L et al. Single-cell DNA amplicon sequencing reveals clonal heterogeneity and evolution in T-cell acute lymphoblastic leukemia. Blood. 2021 Feb 11;137(6):801-811.
  13. ten Hacken E et al. High throughput single-cell detection of multiplex CRISPRedited gene modifications. Genome Biol. 2020;21(1):266. Published 2020 Oct 20.
  14. McMahon CM et al. Clonal selection with RAS pathway activation mediates secondary clinical resistance to selective FLT3 inhibition in acute myeloid leukemia. Cancer Discov. 2019 Aug;9(8):1050-1063.
  15. Jongen-Lavrencic M et al. Molecular minimal residual disease in acute myeloid leukemia. N Engl J Med. 2018 Mar 29;378(13):1189-1199.
  16. Landgren O. MRD testing in multiple myeloma: from a surrogate marker of clinical outcomes to an every-day clinical tool. Semin Hematol. 2018 Jan;55(1):1-3.
  17. Ediriwickrema A et al. Single-cell mutational profiling enhances the clinical evaluation of AML MRD. Blood Adv. 2020 Mar 10;4(5):943-952.
  18. Dillon LW et al. Personalized single-cell proteogenomics to distinguish acute myeloid leukemia from nonmalignant clonal hematopoiesis. Blood Cancer Discov. 2021 May 2:1-7.
  19. Gibson CJ, Steensma DP. New insights from studies of clonal hematopoiesis. Clin Cancer Res. 2018 Oct 1;24(19):4633-4642.
  20. Datlinger P et al. Pooled CRISPR screening with single-cell transcriptome readout. Nat. Methods.14, 297–301 (2017).
  21. Dixit A et al. Perturb-seq: dissecting molecular circuits with scalable single-cell RNA profiling of pooled genetic screen. Cell. 15;167(7):1853-1866.e17 (2016)
  22. Hill AJ et al. On the design of CRISPR-based single-cell molecular screens. Nat. Methods.15, 271 (2018).
  23. Replogle JM et al. Combinatorial single-cell CRISPR screens by direct guide RNA capture and targeted sequencing. Nat Biotechnol. 38, 954-961 (2020).
  24. App Note: Measuring the efficiency of CRISPR genome-editing systems using the Tapestri Platform and Tapestri single-cell DNA custom panels. Mission Bio.
  25. App Note: Tapestri Platform resolves clonality of heterogeneous mouse organoid cancer model through single-cell DNA sequencing of lentiviral barcodes. Mission Bio.
  26. App Note: Single-cell DNA analysis of a novel acute erythroid leukemia (AEL) CRISPR induced mouse model. Mission Bio.
  27. Iacobucci I et al. Modeling and targeting of erythroleukemia by hematopoietic genome editing. Blood. 2021 Jan 19:blood.2020009103.
  28. App Note: In-depth quantification of cell and gene therapy DNA integrations enabled with single-cell sequencing. Mission Bio.
  29. App Note: Deep understanding of cell and gene therapy genome editing protocols enabled with single-cell sequencing. Mission Bio.

Single-cell multiomic analysis stands at the forefront of cancer profiling, offering unparalleled insights into clonal heterogeneity and significantly enhancing our understanding of cancer biology. By overcoming the limitations of traditional methods, this advanced approach paves the way for more precise insights, personalized treatments, and improved patient outcomes.

Are you ready to join the resolution revolution?

Drive Actionable Insights across Cancer Research with Single-Cell Multiomics

References

  1. Lüönd F, Tiede S, Christofori G. Breast cancer as an example of tumour heterogeneity and tumour cell plasticity during malignant progression. Br J Cancer. 2021;125(2):164-175. doi:10.1038/s41416-021-01328-7
  2. Rye IH, Trinh A, Sætersdal AB, et al. Intratumor heterogeneity defines treatment‐resistant HER2+ breast tumors. Mol Oncol. 2018;12(11):1838-1855. doi:10.1002/1878-0261.12375
  3. Marusyk A, Janiszewska M, Polyak K. Intratumor heterogeneity: the Rosetta stone of therapy resistance. Cancer Cell. 2020;37(4):471-484. doi:10.1016/j.ccell.2020.03.007
  4. Aertgeerts M, Demeyer S, Lamote J, et al. Clonal Heterogeneity and Evolution during Treatment in High Hyperdiploid B-Cell Acute Lymphoblastic Leukemia. Blood. 2023;142(Supplement 1):1606. doi:10.1182/blood-2023-180760
  5. Benard BA, Leak LB, Azizi A, Thomas D, Gentles AJ, Majeti R. Clonal architecture predicts clinical outcomes and drug sensitivity in acute myeloid leukemia. Nat Commun. 2021;12(1):7244. doi:10.1038/s41467-021-27472-5
  6. Bianchi G, Ghobrial IM. Biological and Clinical Implications of Clonal Heterogeneity and Clonal Evolution in Multiple Myeloma. Curr Cancer Ther Rev. 2014;10(2):70-79. doi:10.2174/157339471002141124121404
  7. Malone ER, Oliva M, Sabatini PJB, Stockley TL, Siu LL. Molecular profiling for precision cancer therapies. Genome Med. 2020;12:8. doi:10.1186/s13073-019-0703-1
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