Using CRISPR to understand different combinations of gene alterations

Solid tumor bladder cancer is caused by upwards of 5 mutations or more in a single tumor and requires extensive tumor profiling to uncover the most optimal therapeutic path. To better understand which combinations of mutations are important for driving bladder cancer, Dr. John Lee from the Fred Hutch Institute combines sophisticated genome editing, organoid, and mouse model approaches with single-cell technology. In these multi-faceted technology studies, Dr. Lee's lab reveals how different combinations of gene alterations lead to bladder cancer, for a better understanding of the complete disease.