Detecting multiple CRISPR-edited gene modifications

Have you ever wondered what were the exact indels that were made to the DNA after genome editing experiments? Have you wondered what percentage of the cells had certain edits? Have you ever wondered whether the edits were made on only one chromosome or both chromosomes to reveal the zygosity of the edit? Using single-cell DNA sequencing on the Tapestri Platform, the Wu Lab from Dana Farber did just that. Dr. Elisa Ten Hacken shares her recent work using genome editing to create mouse models of chronic lymphocytic leukemia (CLL).